Lactate Testing in Sport: Lactate Thresholds, Testing Protocols and Interpretation of Results
Lactate (lactic acid) is a metabolic product formed during anaerobic glycolysis. For a long time it was thought that lactate is a "fatigue toxin", but modern science has revealed a completely different picture: lactate is an important energy source, signaling molecule and substrate for gluconeogenesis. Accumulation of lactate in the blood is not the cause of fatigue but an indicator that anaerobic energy production exceeds the body's ability to utilize lactate.
First significant increase in lactate above resting level (2 mmol/L). The boundary between light and moderate intensity. Long endurance training sessions take place at this threshold.
Maximal lactate steady state (MLSS) — the highest intensity at which lactate production = utilization (4 mmol/L). The classic "threshold" intensity.
Above LT2 — lactate rises continuously. Intense interval training. It can be maintained for only a few minutes.
Dependence of lactate concentration on exercise intensity. A rightward shift of the curve = improved endurance. The same lactate at a higher power/speed.
| Protocol | Description | Duration | Suitability |
|---|---|---|---|
| Graded Test | Speed/power is increased every 3-5 min., and a blood sample is taken | 30-45 min. | Gold standard — most accurate |
| GXT (Maximal Test) | Continuous increase in load until exhaustion | 8-15 min. | Suitable for measuring VO2max + lactate together |
| Modified Conconi | Shorter graded test with 200m increments every 1-2 min. | 15-20 min. | In field conditions, for runners |
| MLSS (Maximal Lactate Steady State) | Several constant-intensity tests of 30 min. | Several visits | Most accurate determination of LT2, but very time-demanding |
Lactate measurements provide direct metabolic information that cannot be obtained from heart rate or VO2 alone:
Marathon pace is determined (at LT1), half-marathon pace (between LT1-LT2), 10 km pace (at LT2).
FTP (Functional Threshold Power) — power at LT2. Used for defining training zones and race strategy.
Modified protocols in the pool — earlobe blood samples (more easily accessible than fingers during swimming).
Lactate is measured during competition to assess exercise intensity — whether the player is working in the desired intensity range.
To deepen your knowledge about lactate testing in sport, we recommend:
Sports science is constantly evolving. What was considered true 10 years ago may be refuted today by new research. Be curious, read the latest literature, and don't be afraid to experiment — of course, while following safety principles.